Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.13/1351
Título: Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: a case study
Autor: Spínola, Hélder
Bruges-Armas, Jácome
Brehm, António
Palavras-chave: HLA
Polymerase chain reaction - sequence-specific - oligonucleotide probe (PCR-SSOP) technique
Sequence-based typing (SBT) technique
Portugal
Cabo Verde
Guiné Bissau
Azores Islands (Portugal)
.
Data: 2007
Editora: Wayne State University Press
Citação: Spínola, H., Bruges-Armas, J., & Brehm, A. (2007). Discrepancies in HLA Typing by PCR-SSOP and SBT Techniques: A Case Study. Human Biology, 79 (5), 537-543.
Resumo: Six hundred twenty-one samples from Portugal, the Cabo Verde archipelago, and Guinea-Bissau were typed for HLA-A, HLA-B, and HLADRB1usingthepolymerasechainreaction–sequence-specificoligonucleotide probe (PCR-SSOP) method and the sequence-based typing (SBT) method to characterizeandcomparediscrepanciesbetweenthetwomethods.Fifty-three alleles (4.27% of 1,242 chromosomes typed) identified by the PCR-SSOP method were not concordant with the results obtained using the SBT method. Thirty-four (2.74% of total chromosomes typed) PCR-SSOP mistyping results were discrepancies inside the same allele group and 19 others (1.53% of total chromosomes typed) were relative to nonconcordant results between different groups. PCR-SSOP allele mistyping is the result of interpretation difficulties resulting from less intense, absent, or dubious hybridization patterns. Noncommercial PCR-SSOP procedures are highly exigent on the technicians’ experience and the availability of properly calibrated high-precision equipment.
Peer review: yes
URI: http://hdl.handle.net/10400.13/1351
ISSN: 0018-7143
Aparece nas colecções:Artigos em revistas internacionais

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